Figure EV3. Expression of Rab mutants in 293T‐FcR‐K^b and impact of co‐expression of US6 or ICP47 with Rab mutants of the cell surface H2‐K^b and cross‐presentation.

1. H2‐K^b on the cell surface of 293T‐FcR‐K^b co‐expressing US6 or ICP47 along with Rab mutants was measured by flow cytometry.
2. Transient expression of Rab5ACA, Rab22ACA, and Rab7ADN in 293T‐FcR‐K^b was evaluated by immunoblotting with anti‐Myc.
3. Representative plots of at the least three independent experiments of 293T‐FcR‐K^b cells co‐expressing US6 or ICP47 with LacZ or Rab mutants were incubated with opsonized OVA‐coated latex beads for 12 h. The effect of US6 or ICP47 along with the Rab mutants on cross‐presentation was assessed by paraformaldehyde fixation and measuring IL‐2 production by added B3Z cells. The assay was set up in triplicates, and the mean (±SD) of the triplicates is plotted.

Source data are available online for this figure.