Skip to main content
. 2019 Jun 7;38(16):e101168. doi: 10.15252/embj.2018101168

Figure 4. RASSF1A is involved in actin and profilin nuclear export process.

Figure 4

  1. HeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic and nuclear extracts. Lysates from each fraction were probed for actin and profilin alongside GAPDH (as a marker of the cytoplasmic fraction) and Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin and profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments and represent the SEM.
  2. Immunofluorescence images of profilin in control and RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments and represent the SEM.
  3. Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL and siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.
  4. Western blot analysis of actin, profilin, GAPDH and Lamin A/C levels in nuclear and cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin and profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments and represent the SEM.
Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure.