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. 2019 Jul 30;10:976. doi: 10.3389/fpls.2019.00976

TABLE 1.

Primer used in this study.

Primer Name Sequence Note
F1-AGO2 TGGTGAATTGTGAGTT GAATGGTG agl-2 KO
F2-AGO2 ACCCAATTCGCCCTATAGT GAGTCGTGCTGCTGGA TCAAAAGACAT agl-2 KO
F3-AGO2 AAGCCTACAGGACACACA TTCATCGTAGGTACCT GGTCATACCTTCCGCAT agl-2 KO
F4-AGO2 CAGGTCCAAGTCCT GTCCAC agl-2 KO
YG-F CGTTGCAAGACCTGC CTGAA Both KO
PtrpC-F ACGACTCACTATAGGG CGAATTGGGT Both KO
TtrpC-R TACCTACGATGAATGTGTG TCCTGTAGGCTT Both KO
HY-R GGATGCCTCCGCTCGA AGTA Both KO
F1-AGO2-nested GTTTGCAACAATC GCAGGTG agl-2 KO
F4-AGO2-nested TCTCCAACCAG CTACCGATG agl-2 KO
F1-AGO4 TTTGGTCCAGG CCTTGGTTT agl-4 KO
F1-AGO4-nested TTTTCACAACGG GTTTGGGC agl-4 KO
F2-AGO4 ACCCAATTCGCCCTATAGT GAGTCGTGAGCCATTAGCTTGG ATATTCGCA agl-4 KO
F3-AGO4 AAGCCTACAGGACACA CATTCATCGTAGGTAAGTGCCT TCATATCATAATCCTCC agl-4 KO
F4-AGO4 AAGGTTCGTCGGTT GGTAGT agl-4 KO
F4-AGO4-nested CCCTACTTGTCCC ACGTGAT agl-4 KO
F1-COMP-AGO2 ATAATAGCGGCCGCCGGA TAAACTGCCTCTTCGC agl-2 Complementation
F4-COMP-AGO2 TTTTACTGGATCCTCCT GTCCACAATCCCACAA agl-2 Complementation
Ss-Ago2-T7p-1898F TAATACGACTCACTATAG GGAGATGAATCTACA AGCCGTGCTG dsRNA synthesis targeting agl-2
Ss-Ago2-T7p-2065R TAATACGACTCACTATAGG GAGATGAGAGGTA GCCGCTTCATT dsRNA synthesis targeting agl-2
Ss-Ago4-T7p-F TAATACGACTCACTATAGGGAGA TTTGGTTGCAAAATCGATCA dsRNA synthesis targeting agl-4
Ss-Ago4-T7p-R TAATACGACTCACTATAGGGA GATTGCTTGCTTTGTTGACCAG dsRNA synthesis targeting agl-4