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. 2019 Aug 8;10:550. doi: 10.3389/fendo.2019.00550

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Copyright © 2019 Wang, Jin, Zhao and Ni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of melatonin on mitochondrial reactive oxygen species (ROS) accumulation in cells. (A) Detection of ROS content in mitochondria using flow cytometry; (B) Fluorescence intensity of ROS in mitochondria; (C) Quantitative analysis of average fluorescence intensity of ROS in mitochondria: F = 24.903, P < 0.001; control group vs. glutamate injury group: P < 0.001, glutamate injury group vs. melatonin pretreatment group: P < 0.001; **P < 0.01. ns, not significant (n = 6/group).