Table 4.

Laboratory Parameters

Laboratory Parameter	Test
HIV	HIV serostatus is evaluated by ELISA (InTec Products Company, Xiamen, China), and confirmed with an HIV-1/2 western blot (HIV Blot 2.2 WBTM, Genelabs Diagnostics, Singapore). The results of anyone who screens negative for HIV will be confirmed by HIV RNA pooling PCR test (COBAS AmpliPrep /COBAS TaqMan, Roche)
HIV resistance	RNA sequencing
HIV viral load testing	COBAS AmpliPrep /COBAS TaqManv (Roche)
Syphilis	RPR (Shanghai Rongsheng, Shanghai, China) and TPPA (Fujirebio Inc., Tokyo, Japan)
Hepatitis B	HBsAg (Vitros 5600)
HSV-2	IgM-HSV-2, IgG-HSV-2 (Beier Bioengineering, Beijing, China)
Biochemistry	AST, ALT, total bilirubin, creatinine, creatinine clearance, serum phosphate, blood glucose, and lipids (Roche)
Routine blood tests	Full blood count: hemoglobin, leucocytes, platelets; differential count: absolute neutrophil count, absolute lymphocyte count (Mindray BC-5800)
Routine urine tests	Proteinuria (Mindray EH-2080)
Blood drug level testing	LC-MS (AB SCIEX API 6500+)

Abbreviations: ELISA Enzyme-linked immunosorbent assay, RPR Rapid plasma reagin, TPPA Treponema pallidum particle agglutination assay, HSV-2 Herpes simplex virus 2, HBsAg Hepatitis B surface antigen, AST Aspartate transferase, ALT Alanine transferase, LC-MS Liquid chromatography-mass spectrometry