Fig. 6.

CLDN6 inhibits migration, invasion and metastasis of breast cancer through beclin1 in vitro and in vivo. Wound healing and Transwell migration assays showed that beclin1 knockdown drove the migration (scale bar, 200 μm) and invasion (scale bar, 50 μm) of DPN-treated (a) and CLDN6-overexpressing MDA-MB-231 cells (b). c IVIS imaging was performed to show lung metastatic sites. Representative H&E staining of lung sections from the three groups (n = 5). d The number of mice with lung metastasis was counted in each group. e The number of lung nodules in each group. f Representative images of liver metastasis and H&E staining in the three groups (n = 5). Black arrowheads indicate the metastatic sites. g The number of mice with liver metastasis was counted in each group. h The number of liver nodules in each group. Data are presented as mean ± SD. The data shown are representative results of three independent experiments. *P < 0.05, **P < 0.01