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. 2019 Aug 14;19:810. doi: 10.1186/s12885-019-5998-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effect of GPR64 loss on cell growth in human endometrial cancer cells. a and b The expression of GPR64 mRNA and protein in Ishikawa (a) and HEC1A (b) cells transfected with non-targeting pool (NT) siRNA or GPR64 siRNA was examined by RT-qPCR and Western blot analysis, respectively. c and d Colony formation assay of Ishikawa (c) and HEC1A (d) cells transfected with NT siRNA or GPR64 siRNA. Samples from each treatment were transferred to flat-bottomed 24-well plates and incubated. Cells were fixed and stained with crystal violet. The average colony formation number was quantified with crystal violet stained cells. e and f Cell proliferation assay of Ishikawa (e) and HEC1A (f) cells transfected with NT siRNA or GPR64 siRNA. The results represent the mean ± SEM. *, p < 0.05; **, p < 0.01; and ***, p < 0.001