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. 2019 Aug 14;19:810. doi: 10.1186/s12885-019-5998-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The reduction of Cx43 levels by GPR64 loss through AMPK activation in Ishikawa cells. a The Western blot analysis of phospho-AMPK^Thr172, Total AMPK, and Cx43 proteins in Ishikawa cells transfected with NT siRNA or GPR64 siRNA on day 1 and day 6. Actin was used as sample-loading control. b Quantification of western blot analysis of Cx43. c The expression of Cx43 gene during in vitro culture after GPR64 siRNA transfection. Cx43 was significantly decreased on day 3 and day 6 in cells transfected with GPR64 siRNA. The results represent the mean ± SEM. *, p < 0.05; and **, p < 0.01