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. 2019 Aug 15;16:102. doi: 10.1186/s12985-019-1204-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Competitive ELISA using synthetic peptides. The recombinant DENV-1 NS1 proteins (20 μg/mL) were coated on an ELISA plate. Aliquots (2 μg/well) of each antibody (Membrane-1, Membrane-2, AuNPs-1 and AuNPs-2) were mixed with each peptide (500 μg/well) corresponding to the amino acid positions shown in the left panel. Peroxidase-conjugated anti-mouse IgG was used to detect antibodies bound to NS1 protein. Abs. indicates the optical density at 650 nm. P1: peptide-1, P2: peptide-2, P3: peptide-3, P4: peptide-4, P5: peptide-5, P6: peptide-6, and P7: peptide-7. PC: positive control (run without added synthetic peptide)