Table 1.

Target variables used for the screening of culture parameters for the osteogenic differentiation of hASCs. hASC source and seeding density were chosen as factors related to the stromal cells; base medium, serum, L-ascorbate-2-phosphate, ß-glycerophosphate, dexamethasone, and BMP-9 were chosen as factors related to the medium. The factor variability was set between a certain range based on the values commonly used in literature

Variable	Description	Range of variability from (-) to (+)			References
V1	hASC source	ZB	EFS		[14]
V2	Cell seeding density, per well	2000	6000	10000	[5]
V3	Base culture medium	DMEM/F-12	DMEM		[15]
V4	Serum source	5% hPL	10% FBS		[8]
V5	L-ascorbate-2-phosphate	0	50 μM		[16]
V6	ß-glycerophosphate	0	10 mM		[16]
V7	Dexamethasone	0	100 nM		[16]
V8	Bone morphogenetic protein-9	0	100 ng/mL		[18]