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. 2019 Aug 8;12:188. doi: 10.3389/fnmol.2019.00188

Figure 6.

Figure 6

Transfection of mimic and inhibitors of miR-30 family miRNAs in GL261 cells indicates the regulation of several genes by these miRNAs. (A) mRNA expression of Ptk9 with the use of positive (miR-1) mimic as compared to the negative mimic in GL261, Neuro-2a, and NIH/3T3 cells. It also presents mRNA expression of Hmga2 with the use of positive inhibitor (against let-7c) as compared to the negative inhibitor in all the three cell lines. Gapdh was used as a normalization control; n = 3 samples in each group, two-sample t-test, p = 0.058, 0.124 and 0.163, respectively for the positive mimic transfection and p = 0.011, 0.194 and 0.059, respectively for positive inhibitor transfection, data is represented as Mean ± SEM. (B) Aligned sequences of mature miR-30 miRNAs, where highlighted box shows seed region from position number 2–9 that is fully conserved in all the family members. (C) Fold change in mRNA expression of probable gene targets upon manipulation of miR-30c levels in GL261 cells by transfecting with its mimic and inhibitor. The expression data of these genes are analyzed with respect to their expression levels in cells transfected with negative mimic and negative inhibitor, respectively. Gapdh was used as a normalization control; n = 3 samples in each group; p = 0.352, 0.055, 0.054, 0.017, 0.006, 0.027, 0.020, 0.007, 0.014, 0.159, 0.026 and 0.832, respectively in miR-30c mimic transfections and p = 0.679, 0.605, 0.157, 0.164, 0.022, 0.030, 0.050, 0.101, 0.218, 0.055, 0.069 and 0.873, respectively in miR-30c inhibitor transfections, data represented as Mean ± SEM. (D) Fold change in mRNA expression of probable gene targets upon transfection of mimic and inhibitor of miR-30 miRNAs. The expression data of these genes are analyzed with respect to their expression levels in cells transfected with negative mimic and negative inhibitor, respectively. Gapdh was used as a normalization control; n = 3 samples in each group, p = 0.507, 0.111, 0.538, 0.223, 0.005, 0.017, 0.003, 0.227, 0.042, 0.585, 0.631 and 0.003, respectively in miR-30e mimic transfections and p = 0.821, 0.124, 0.18, 0.248, 0.006, 0.197, 0.021, 0.06, 0.091, 0.292, 0.019 and 0.99, respectively in miR-30e inhibitor transfections, data represented as Mean ± SEM. *p ≤ 0.05, **p ≤ 0.01.