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. 2019 Aug 8;12:188. doi: 10.3389/fnmol.2019.00188

Figure 7.

Figure 7

Relative luciferase activity in HEK-293 cells transfected with various 3′UTR clones along with miR-30 mimics and inhibitors. HEK-293 cells were transfected with luciferase reporter constructs having different 3′UTR clones, Rcor1 (A), Socs3 (B), Omg (C), Khnyn (D), Ppp3r1 (E) and Mll3 (F). Luciferase activity readings of different transfection sets were normalized with their respective negative mimics. Transfection with miR-124 was also used as a negative control. All the experiments were carried out thrice in triplicates; two-sample t-test, p = 0.366, 0.165, 0.492, 0.042 and 0.25, respectively for Rcor1 3′UTR, p = 0.278, 0.0009, 0.063, 0.0017 and 0.0008, respectively for Socs3 3′UTR, p = 0.472, 0.10, 0.301, 0.143 and 0.109, respectively for Omg 3′UTR, p = 0.305, 0.064, 0.539, 0.081 and 0.191, respectively for Khnyn 3′UTR, p = 0.823, 0.0265, 0.238, 0.014 and 0.0006, respectively for Ppp3r1 3′UTR, p = 0.366, 0.033, 0.229, 0.039 and 0.045, respectively for Mll3 3′UTR. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.