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. 2019 Jun 21;2(3):172–185. doi: 10.1089/crispr.2019.0009

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© Nicholas C. Huston, et al. 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 3. — Mismatch permissiveness is guide intrinsic. The average mismatch tolerance of several guides on randomized mismatches varies widely when determined as a function of mismatch number. Reactions were run at 10:1 RNP:target library for 30 min. (A) A guide-intrinsic mismatch permissiveness (GIMP) score can be derived from this function by integrating over mismatch number (indicated by the shaded region). (B) The GIMP score varies widely among different guides. A high GIMP score indicates high intrinsic permissiveness of a guide to cleave indiscriminately. Low GIMP guides are intrinsically specific.