Expression analysis of NDRG1. (A) Phylogenetic tree of NDRG1 proteins constructed with MEGA6 software. Protein sequences of NDRG1 from different species were taken from GenBank, under accession numbers XP_020944534 (Sus scrofa), NP_001030181 (Bos taurus), NP_001011991 (Rattus norvegicus), NP_032707 (Mus musculus), NP_001128714 (Homo sapiens), XP_007999783 (Chlorocebus sabaeus), XP_005628031 (Canis lupus familiaris), XP_012820024 (Xenopus tropicalis), XP_019315342 (Panthera pardus), XP_017730622 (Rhinopithecus bieti), XP_012397285 (Sarcophilus harrisii), XP_005935015 (Haplochromis burtoni), XP_005564176 (Macaca fascicularis), XP_011793534 (Colobus angolensis
palliatus), XP_008321638 (Cynoglossus semilaevis), XP_007106495 (Physeter catodon), XP_023104387 (Felis catus), XP_010586814 (Loxodonta africana), XP_009454233 (Pan troglodytes), and XP_007605917 (Cricetulus griseus). (B) NDRG1 mRNA levels were detected in porcine tissues by RT-qPCR. Values were normalized to the β-actin (ACTB) mRNA levels. Relative amounts of NDRG1 mRNA were compared with those in the liver. Data represent means ± standard errors of the means from three independent experiments.