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. 2019 Aug 13;93(17):e00526-19. doi: 10.1128/JVI.00526-19

TABLE 1.

Primers used for gene cloning and RT-qPCR

Primer Sequence (5′–3′) Product size (bp)
Flag-NDRG1 GGGGTACCAATGTCTCGGGAGCTGCAGGA 1,155
CGGGATCCTTAGCAGGACACCTCCGCAGA
Q-Sus NDRG1 ACACCTACCGACAGCACATC 177
CGTCCACAGCAGGAGAATTG
Q-sus β-actin CTGAACCCCAAAGCCAACCGT 317
TTCTCCTTGATGTCCCGCACG
Q-PRRSV ORF7 AGATCATCGCCCAACAAAAC 144
GACACAATTGCCGCTCACTA
Q-Chlorocebus β-actin CGTGGACATCCGTAAAGAC 182
GGAAGGTGGACAGCGAGGC
Q-Chlorocebus ACC TAGTCTGCCACGGATCCAGA 179
GGGAGGGATCTCTGAGGGTT
Q-Chlorocebus FASN CACATCGTTCGAGCAGCATG 133
AATTTCCAGGAAGCGACCGT
Q-Chlorocebus SCD AGGGCCCCAATGTATGTGTG 196
AAAGATGTAAGGCACCCGGG
Q-Chlorocebus ATGL GAGATGTGCAAGCAGGGCTA 120
ACTCTCCATGGCCTCATCCT
Q-Chlorocebus HSLL CCTCCGGGAGTATGTTACGC 113
ACACCAGCCCAATGGAGATG
Q-Chlorocebus MGL GTCTTCCTTCTGGGCCACTC 159
GTTGAGCACTTTCGCAGCAA
Q-Chlorocebus NDRG1 ACGCACGAACACACAAACGG 135
TCACCTCAGCGAGGTCTACG