FIG 7.

Whole-organoid expression of early NPC markers is not significantly changed with HCMV infection. (A) Whole-organoid RNA was isolated at 14 dpi from mock- and HCMV TB40/E-eGFP-infected day 30 organoids treated with vehicle (V) or 10 μM MBV. Three separate organoids were analyzed for each condition. Expression of Sox2 and Pax6 was assessed using RT-PCR relative to results with the GAPDH control. (B) Average signal intensity of Sox2 and Pax6 relative to that of GAPDH from three separate organoids is shown. ns, not significant.