TABLE 2.

Variable region characteristics of AIIMS_329 and AIIMS_330 in the time frame of 90 to 117 months of age^a

Twin and age (mo)	No. of sequences (SGA amplicons)	V1		V2		V3		V4		V5
		Length	No. of PNGS	Length	No. of PNGS	Length	No. of PNGS	Length	No. of PNGS	Length	No. of PNGS
AIIMS_329
90	26	26	2	42	2	36	1	30	3	16	2
112	24	28	5	56	2	36	1	37	6	12	2
117	29	40	5	50	2	36	1	38	5	12	1
P value		****	****	****				****	****
AIIMS_330
90	24	24	4	44	3	37	1	37	5	17	2
112	20	23	4	55	5	37	1	36	3	17	2
117	21	34	4	59	5	37	1	36	5	16	2
P value		****		****	****

^aThe variable loop length (in nucleotides) and number of PNGS of longitudinal SGA-amplified amplicons of AIIMS_329 and AIIMS_330 were calculated using the Variable Region Characteristics tool available at the HIV Database (https://www.hiv.lanl.gov/content/sequence/VAR_REG_CHAR/index.html). The Kruskal-Wallis test was used to compare the difference in variable loop length and number of PNGS of the SGA amplicons in the time frame of 90 months to 117 months. Given are the average loop length and PNGS of all SGA amplicons for the respective time point. ****, P < 0.0001.