FIG 6.

Detection of Gag-Gag interactions by NanoBRET. HEK293T cells were cotransfected with expression vectors encoding Gag-HaloTag and its corresponding Gag-NanoLuc (43). At 48 h posttransfection, NanoBRET signals were measured, and values relative to that for control NL4-3 are presented (n = 4; means ± SE). NanoBRET data were normalized by subtracting the signal value for a Gag CA linker/CTD deletion mutant (deletion of CA amino acids 146 to 231). Significance relative to control NL4-3 was determined by Welch’s t test. **, P < 0.01; *, P < 0.05. A black bar, red bars, and blue bars indicate NL4-3, linker mutants, and MHR mutants, respectively.