FIG 2.

Virus stability in NK solution with different amounts of K⁺, Mg²⁺ (M), and Ca²⁺ (C) at 37°C. (A) Fluorescence measurements of SGII dye in the presence of 1 μg E1 in NKMC, containing 30 mM K phosphate (black lines), NKMC with higher, 60 mM, K phosphate (red lines), and NK (i.e., without Mg²⁺ and Ca²⁺, blue lines) solutions. (B) Fluorescence measurements of SGII dye in the presence of 1 μg E1 in NK solution with different amounts of CaCl₂ (C = 200 μM, 0.1C = 20 μM, 0.01C = 2 μM). (C) Fluorescence measurements of SGII dye in the presence of 1 μg E1 in NK solutions with different amounts of MgCl₂ (M = 500 μM, 0.1M = 50 μM, 0.01M = 5 μM). (D) Fluorescence measurements of SGII dye at 37°C in the presence of 1 μg E1 in either intra- and extracellular Na⁺ and K⁺ concentrations, combined with intracellular 800 μM MgCl₂ and 0.2 μM CaCl₂ concentrations. (E and F) Fluorescence measurements of SGII dye at 37°C in the presence of 1 μg E1 in various Na⁺ and K⁺ concentrations between intra- and extracellular values, but constant intracellular 800 μM MgCl₂ and 0.2 μM CaCl₂ concentrations.