TABLE 2.
Summary of cryoEM data collection, refinement, and validation statistics
| Category | Value |
|
|---|---|---|
| Treated E1 | Control E1 | |
| Data collection | ||
| Voltage (kV) | 200 | 200 |
| Electron exposure (e−/Å2 × s) | 30 | 30 |
| Pixel size (Å) | 1.24 | 1.24 |
| No. of micrographs | 1,246 | 979 |
| Reconstruction | ||
| No. of particles | 14,615 | 45,309 |
| B factor (Å2) | −70 | −70 |
| FSCa threshold | 0.143 | 0.143 |
| Resolution (Å) | 3.6 | 3.5 |
| Model building (aa)b | ||
| VP1 (full protein, 281 aa) | 55–130 and 137–280 | 1–281 |
| VP2 (full protein, 261 aa) | 12–54 and 58–261 | 7–261 |
| VP3 (full protein, 239 aa) | 3–174 and 184–237 | 1–239 |
| VP4 (full protein, 68 aa) | none | 1–14 and 22–68 |
| Model validation | ||
| MolProbity score (percentile) | 1.25/100thc | 1.08/100thd |
| Ramachandran outliers (%) | 4.3 | 2.06 |
| Poor rotamers (%) | 1.7 | 1.24 |
| Clash score | 0 | 0 |
a
FSC, Fourier shell correlation.
b
aa, amino acid.
c
N = 342, 3.25 Å to 3.85 Å.
d
N = 27,675, 0 Å to 99 Å.