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Pathogenicity in mice. Groups of 15 BALB/c mice were inoculated with 200,000 PFU of virus in 40 μl or vehicle (PBS). (A) Mean ± standard deviation (SD) lung viral titers on day 2 postinfection (n = 5) were titrated by plaque assay on MDCK cells. (B) Mean (± standard error of the mean [SEM]) percent weight change. One-way ANOVA with Tukey’s posttest was used to compare WT virus to the other viruses. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
