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vPK increases ZEBRA expression through induction of serine 824-phosphorylated KAP1. HH514-16 cells were transfected with combinations of the indicated plasmids (A) or siRNA plus plasmids (B). Transfected cells were treated with NaB 24 h later and harvested at different time points posttreatment for immunoblotting with the indicated antibodies. Numbers reflect relative amounts of ZEBRA and vPK after normalization to β-actin. All experiments were performed at least twice.
