Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Candidate chemicals selected using the rPR8-GFP virus. By a plaque reduction assay in MDCK cells (96-well plate; 4 × 104 cells/well), eight candidate chemicals were selected based on the GFP expression of oseltamivir-treated wells. A total of 100 μg of each chemical dissolved in DMSO was serially 2-fold diluted in PBS and added to overlaying agar. The multiplicity of infection of rPR8-GFP was 0.5, and GFP expression was observed at 24 h postinfection. PBS was used for mock infection.
