Fig. 4.

Methylxanthines induced DAF-16/FOXO and SKN-1 translocation to the nucleus and regulated target genes. A, three levels to determine the pattern of the DAF-16::GFP subcellular translocation. B, methylxanthines induced significant translocation of DAF-16::GFP. C. Two patterns of the SKN-1::GFP subcellular translocation. D, methylxanthine treatment induced a significant translocation of SKN-1::GFP in LD1. Data are presented as mean ± SEM, experiments were repeated at least three times, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001, and compared to the untreated control by one-way ANOVA following Dunnett.