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. 2019 Aug 15;15(8):e1008318. doi: 10.1371/journal.pgen.1008318

Fig 2. Uro knockdown enhances UA concretion formation on a high yeast diet.

Fig 2

(A) Mass spectrometric analysis of purine metabolite concentrations of DaGS>Uro-RNAi #1 fly homogenates comparing siblings fed the HY + RU and HY - RU diet for 14 days. (B) Micro-CT images of intact flies from (A). Fly length is ~2 mm. (C) Guts with the attached Malpighian tubules were dissected from flies in (B). Black arrowheads point to solid aggregates (concretions) in the hindgut region. (D) Light microscopic pictures of concretions extracted from the hindgut lumen of DaGS>Uro-RNAi #1 flies fed the HY diet + RU for 14 days. (E) Metabolomic analysis of the same purine metabolites as in (A) of extracted concretions from Uro knockdown flies. (F) The extent and kinetic of concretion formation in DaGS>Uro-RNAi #1 flies fed the LY or HY diet +/- RU for 4 to 14 days was determined by microscopic analysis after dissection of the hindgut. (G) Concretion formation of flies from Fig 1E fed the LY or HY diet for 14 days was determined by microscopic analysis after dissection of the hindgut. Error bars represent the SE of multiple biological repeats. Further supporting information is shown in S2 Fig.