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. 2019 Aug 15;15(8):e1008318. doi: 10.1371/journal.pgen.1008318

Fig 6. NOX mediated ROS production triggers lifespan attenuation and UA concretion formation on a high yeast diet.

Fig 6

(A) Levels of NOX mRNA upon modulation of FOXO. Recombinant DaGS::Uro-RNAi #1 flies were crossed to w1118 (w; no additional UAS-locus), or active UAS-transgenes triggering either over-expression of FOXO or inhibition of NOX (NOX-RNAi) and fed the HY + RU or HY - RU diet before relative mRNA levels of urate oxidase (Uro) and NADPH oxidase (NOX) were determined by qRT-PCR. (B) Concretion formation of recombinant DaGS::Uro-RNAi #1 flies crossed to w1118 or one of the two NOX-RNAi lines after 14 days of feeding the HY + RU diet. (C) Relative purine metabolite levels of fly homogenates from recombinant DaGS::Uro-RNAi #1 flies crossed to w1118 or NOX-RNAi #1. Metabolite levels were compared after 14 days of feeding flies the HY + RU or HY - RU diet. (D) Survival curves of flies from (C). Error bars represent the SE of multiple biological repeats. Supporting data is shown in S4 Fig and S5 Fig.