Table 2. Dietary supplementations and drug treatments.
Dietary Additive | Ingredients | Vendor | Concentrations |
---|---|---|---|
10 mM Pu (Purines) | Adenine, Guanine | Sigma-Aldrich | 5 mM each |
20 mM Pu | Adenine, Guanine | Sigma-Aldrich | 10 mM each |
40 mM Pu | Adenine, Guanine | Sigma-Aldrich | 20 mM each |
20 mM Py (Pyrimidines) | Cytosine, Thymine | Sigma-Aldrich | 10 mM each |
AP | Allopurinol | Sigma-Aldrich | 1, 5, 10 mM |
MTX | Methotrexate | Sigma-Aldrich | 0.2, 1, 5, 50 μM |
Vit C | Vitamin C | Sigma-Aldrich | 10, 50, 100 mM |
ACY | Apocynin | Sigma-Aldrich | 1, 5 mM |
5% Protein | BSA, Casein | Cell Signaling Technology, Sigma-Aldrich | 2.5% (w/v) each |
15% Protein | BSA, Casein | s. above | 7.5% (w/v) each |
10% Sucrose | Sucrose | Mallinckrodt Chemicals | 10% (w/v) |
20% Sucrose | Sucrose | s. above | 20% (w/v) |