Table 1. Proteins and their encoding genes, sequences, codes, and primers.
| Protein | Gene symbol | Sequence | Primer sequence |
|---|---|---|---|
| Collagen type I alpha 2 chain | col1α2 | NM_001079714.2 | F: AAACCAGGCGAAAGGGGTCT |
| R: GATGGACCACGGCTTCCAAT | |||
| Collagen type II alpha 1 chain | col2α1 | NM_204426.1 | F: GACCGCGACCTCCGACAA |
| R: CCTCGGGGTCCTACAACATC | |||
| Collagen type X alpha 1 chain | col10α1 | XM_003641007.3 | F: CCACAACATTTGAGGACGGA |
| R: CCCCTTGATGCTGGACTGTT | |||
| Osteopontin (secreted phosphoprotein 1) | spp1 | NM_204535 | GAAAAATACGACCCCAGGAGC |
| TGCTGAAGTGAAGCCAGGTC | |||
| Osteonectin (secreted protein acidic and cysteine rich) | sparc | NM_204410.1 | ACTGCACCACTCGCTTCTTT |
| ATGTCCTGCTCCTTAATGCCAA | |||
| Osteocalcin (bone gamma-carboxyglutamate protein) | bglap | NM_205387.1 | TAAAGCCTTCATCTCCCACCG |
| TCAGCTCACACACCTCTCGT | |||
| Alkaline phosphatase | alpl | NM_205360.1 | GTCAAAGCCAACGAGGGGAC |
| TTCATCCTTAGCCCAGCGGA | |||
| ß-actin | bact | NM_205518 | TGATATTGCTGCGCTCGTTG |
| ATACCAACCATCACACCCTGA |