Table 1. Statistics for data collection and processing, structure solution and refinement of the complexes composed of the ACBD3 GOLD domain and 3A proteins of enterovirus A71, poliovirus 1, enterovirus D68, and rhinovirus B14.

Numbers in parentheses refer to the highest resolution shell of the respective dataset. R.m.s.d., root-mean-square deviation.

Crystal	GOLD + EVA71 3A	GOLD + PV1 3A	GOLD + EVD68 3A	GOLD + RVB14 3A
Construct	fusion protein	L24A mutant	wild type	wild type
PDB accession code	6HLW	6HLV	6HLN	6HLT
Data collection and processing
Space group	P 21 21 21	C 1 2 1	C 1 2 1	P 1 2 1
Cell dimensions—a, b, c (Å)	46.4, 54.9, 208.7	90.7, 53.8, 62.8	96.9, 55.9, 64.5	54.4, 79.0, 70.6
Cell dimensions—α, β, γ (°)	90.0, 90.0, 90.0	90.0, 107.6, 90.0	90.0, 112.1, 90.0	90.0, 112.4, 90.0
Resolution at I/σ(I) = 2 (Å)	2.80	2.75	2.27	2.90
Resolution range (Å)	48.61–2.73 (2.83–2.73)	43.20–2.50 (2.59–2.50)	47.45–2.10 (2.18–2.10)	42.44–2.82 (2.92–2.82)
No. of unique reflections	14,873 (1,413)	10,009 (983)	18,521 (1,848)	13,308 (1,286)
Completeness (%)	99.62 (98.95)	98.89 (98.89)	98.34 (99.09)	98.38 (95.33)
Multiplicity	5.0 (5.1)	3.4 (3.5)	3.8 (3.8)	3.8 (3.7)
Mean I/σ(I)	12.80 (1.62)	7.43 (1.24)	10.78 (1.21)	9.85 (1.61)
Wilson B factor (Å2)	72.87	55.88	47.36	57.90
R-merge / R-meas (%)	7.76 / 8.67	11.71 / 13.98	7.61 / 8.93	10.97 / 12.78
CC1/2	0.998 (0.730)	0.993 (0.542)	0.997 (0.556)	0.995 (0.665)
CC*	1.000 (0.919)	0.998 (0.838)	0.999 (0.845)	0.999 (0.894)
Structure solution and refinement
R-work (%)	23.88 (36.97)	21.50 (30.46)	22.07 (36.93)	21.04 (31.80)
R-free (%)	25.68 (44.64)	24.14 (32.46)	25.08 (37.07)	23.94 (37.05)
R.m.s.d.—bonds (Å) / angles (°)	0.003 / 0.73	0.003 / 0.82	0.007 / 1.06	0.008 / 1.02
Average B factors (Å2)	75.8	52.0	47.7	55.2
Clashscore	0.96	0.74	1.08	0.55
Ramachandran favored/outliers (%)	98 / 0	99 / 0	100 / 0	98 / 0