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. 2019 Aug 5;15(8):e1008136. doi: 10.1371/journal.pgen.1008136

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© 2019 Li et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A, B) HUG1 deletion, in combination with CRT1 deletion, shows additive suppression effects on mck1Δ (A) and mck1Δdun1Δ (B), but not on dun1Δ (B). Yeast strains with the indicated genotype (S1 Table) were tested for the HU sensitivity by serial dilution analysis as described in Fig 1A. (C) HUG1 deletion does not reduce SML1 transcription. RT-PCR analysis of SML1 mRNA in hug1Δ with or without HU treatment for 3 h. (D) Hug1 acts as a protein. A strain carrying TAG-HUG1, with its start codon (ATG) replaced by a stop codon (TAG) was tested in parallel with hug1Δ for the HU sensitivity in the indicated genotypes as described in (A).