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. 2019 Aug 5;15(8):e1008136. doi: 10.1371/journal.pgen.1008136

Fig 6. Mck1 regulates the transcription of HUG1 in a Crt1-independent way.

Fig 6

(A) Mck1 and Crt1 co-regulate the Hug1 protein level. Cells were grown and treated as Fig 4C. Hug1-13Myc and Tubulin were immunoblotted with anti-Myc and anti-tubulin antibodies, respectively. (B, C) RT-PCR analysis of the HUG1 mRNA levels relative to ACT in various alleles after HU treatment for 3 h (B) or the indicated time points (C). The value of untreated WT was set to 1.0. (D) Combinational deletion of SML1, CRT1 and HUG1 displays additive suppression effects on mec1Δ and mck1Δdun1Δ. Yeast strains with the indicated genotype (S1 Table) were tested for the HU sensitivity by serial dilution analysis as described in (A).