. 2019 Aug 5;15(8):e1007992. doi: 10.1371/journal.ppat.1007992

Table 1. The amino acid differences between GI and GIII JEVs.

Viral proteins	Consensus substitutions of GI JEV viral proteins^a	Strain-specific substitutions
Capsid	K70R, R100K, G110S, V120I, I122T	K10R^f, Q102R^d,
prM	T57A, M/A58V,	M44L^d, S90T^d, V140T^e/A^f, N148T^b^,^c, N149T^b^,^c/S^e^,^f
E	T129M, A222S, S327T, A366S,	S123N^f, K209R^b^,^c, V385I^d
NS1	K51Q, A70S, H147R, Y206L, K251R, V298I	S175N^f,
NS2A	V6I, T97A, S149T, T151A, K187R	S70N^f, V154I^f,
NS2B	E55D, D65E, V99L	K78N^e, D81E^b^,^c^,^d
NS3	S14L, A78S, A105P, E177D, N182S, R185K, E354D	K15R^f, I109F^b, E122K^c, V123I^e, Q188R^f, R218K^d, R237K^e
NS4A	I110V
NS4B	K20R, N73S, A118V	S24P^f, F88S^d, A205R^d, A216R^d
NS5	E22D, R101K, K280R, K287R, A372V, D429G, D438N, R432L, E588G, V878I	A338V^e, Y^b^,^c^,^d/H386H^e^,^f, W751C^b^,^c, D776Y^d

^aA total of 71 GIII and 77 GI viruses were analyzed by VisSPA software. These conserved substitutions were observed for 70% or 95% of GIII and GI JEVs used in this study, and were respectively highlighted in italic or boldface. Substitutions resulting in an altered charge were underlined.

^bGIII CH1392 strain-specific substitutions

^cGIII T1P1 strain-specific substitutions

^dGIII RP9 strain-specific substitutions

^eGI YL209-4 strain-specific substitutions

^fGI TC2009-1 strain-specific substitutions