Fig 6. MBD9 is not a core subunit of the Arabidopsis SWR1 complex.

(A) Schematic representation of the Arabidopsis SWR1 protein complex based on TAP-tag experiments, including MBD9 as a potential SWR1 subunit. (B) Estimated size of the Arabidopsis SWR1 complex with and without MBD9 as a subunit. The estimated size was calculated based on the known stoichiometry of the yeast SWR1 complex [59] and predicted molecular weights of the Arabidopsis SWR1 subunits listed in Table 1. (C) Protein gel blots of even-numbered SEC fractions from WT (top blot) and mbd9-1 (bottom blot) plants. The blots were incubated with the ARP6 monoclonal antibody [40]. Asterisks indicate the ARP6 peak fractions. The average molecular weights of ARP6-containing protein complexes in WT and mbd9-1 plants were calculated from two biological replicates and presented on the right side of the corresponding protein blots.