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. 2019 Aug 5;15(8):e1008326. doi: 10.1371/journal.pgen.1008326

Table 1. Arabidopsis thaliana homologs of SWR1-C subunits co-purify with N-TAP ARP6 and C-TAP ARP6.

IP-ed proteins Gene ID Predicted MW (kDA) Peptide number (N-TAP 11–4) Peptide number (N-TAP 1–2) Peptide number (C-TAP 10–2)
ARP6
PIE1
SWC2
SWC4
SWC6
RuvB1
RuvB2
HTA8
HTA9
HTA11
HTB2
HTB4
HTB9
ARP4
ACT1
YAF9a
YAF9b
MBD9
TRA1A
TRA1B
AL5
AL6
AL7
AT3G33520
AT3G12810
AT2G36740
AT2G47210
AT5G37055
AT5G22330
AT5G67630
AT2G38810
AT1G52740
AT3G54560
AT5G22880
AT5G59910
AT3G45980
AT1G18450
AT2G37620
AT5G45600
AT2G18000
AT3G01460
AT2G17930
AT4G36080
AT5G20510
AT2G02470
AT1G14510
46.9
234
42.1
49.8
19.1
50.3
52.1
14.4
14.3
14.5
15.7
16.4
16.4
48.9
41.8
30.2
30.4
240.4
436.3
433.6
29.6
28.8
28.3
34
28
5
7
2
25
23
ND
2
ND
6
8
8
14
9
1
ND
12
18
17
3
4
3
35
12
3
5
3
22
22
ND
ND
1
ND
5
ND
11
9
ND
ND
4
1
1
2
2
ND
28
31
3
5
3
21
23
ND
ND
3
5
5
5
13
8
6
ND
6
15
11
2
2
3

We used mass spectrometry (MS) analysis to identify eluted proteins co-purified with ARP6-TAP-tag. All proteins identified by MS from three independent experiments (S1 Table) were first filtered against the proteins purified from WT by TAP and then against the list of 760 known non-specific binders of the GS-TAP-tag. Table 1 contains the proteins that are not found on the list of the TAP-tag background proteins and are known homologs of SWR1 complex subunits in yeast and SRCAP subunits in mammals. The table also includes proteins such as MBD9, TRA1A and TRA1B, and Alfin1-like family proteins (bottom six rows) that may represent novel subunits of the SWR1 complex. The table shows the expected MWs for each identified protein, and the total number of peptides identified by MS from two N-TAP-ARP6 and one C-TAP-ARP6 transgenic plants. ND = not detected.