Table 2. Comparison of BRAF and NRAS results by allele specific amplification, Sanger sequencing, IHC and Idylla testing.

		BRAF IHC				BRAF Idylla
		N	P	NT	All	No mutation	BRAF V600	Insufficient material	Cartridge failure	All
BRAF ASA +Sanger sequencing	WT	38	0	4	42	41		1		42
	BRAF V600X*	0	1	0	1	1				1
	BRAF V600E	3	13	1	17		17			17
	BRAF V600K	5	0	0	5		4		1	5
	All	46	14	5	65	42	21	1	1	65
		NRAS Idylla
		WT	NRAS Q61X	NRAS G13X	Insuff. material		Cartridge failure	All
NRAS Sanger sequencing	WT	20					20
	NT (BRAF mutated)	22				1	23
	NRAS Q61R	2	7				9
	NRAS Q61K		5				5
	NRAS Q61L	1	2				3
	NRAS Q61_E62delinsHK	1					1
	NRAS G13R			1			1
	NRAS G60E	1					1
	Not Contributive	1			1		2
	All	48	14	1	1	1	65

N, Negative; P, Positive; NT, Not Tested

* Nucleotide characterisation by Sanger sequencing failed