Table 1.
Schematic of FANA-DNA ASO Gapmer and Oligonucleotide Sequences Used in This Study
| ASO | Sequences (5′ > 3′) | Length | DNA Gap Length |
|---|---|---|---|
| DIS-FANA-G0 | UGCCGUGUGCACUUCAGCAA | 20 | 0 |
| DIS-FANA-G1.1 | UGCCGUGUgCACUUCAGCAA | 20 | 1 |
| DIS-FANA-G1.2 | UGCCGUGUGcACUUCAGCAA | 20 | 1 |
| DIS-FANA-G2 | UGCCGUGUgcACUUCAGCAA | 20 | 2 |
| DIS-FANA-G4 | UGCCGUGtgcaCUUCAGCAA | 20 | 4 |
| DIS-FANA-G6 | UGCCGUgtgcacUUCAGCAA | 20 | 6 |
| DIS-FANA-G9 | UGCCGtgtgcacttCAGCAA | 20 | 9 |
| DIS-DNA | tgccgtgtgcacttcagcaa | 20 | – |
| tat/rev-FANA | UGAGCtcttcgtcgCUGUCU | 20 | 9 |
| U5-FANA | GUCUGAgggatctctAGUUAC | 21 | 9 |
| Cy3-FANA | Cy3-UGAGCtcttcgtcgCUGUCU | 20 | 9 |
| Cy3-DIS-FANA-G0 | Cy3-UGCCGUGUGCACUUCAGCAA | 20 | 0 |
| Cy3-DIS-DNA | Cy3-tgccgtgtgcacttcagcaa | 20 | – |
| SC-FANA | UGACCCUAtgctgtUCCUAUA | 21 | 6 |
| GAPDH-FANA | CAAGCUtcccgttcUCAGCC | 20 | 8 |
| CpG 2395 | tcgtcgttttcggcgcgcgccg | 22 | – |
Lowercase letters represent 2′-deoxyribonucleotides; bold uppercase letters represent 2′-fluoroarabinonucleosides. Backbones are uniformly modified with phosphorothioate (PS) linkages. Cy3 is linked to the 5′ end of Cy3-FANA through a PS linkage.