Table 2.

Oligonucleotide primer sequences and PCR conditions of the target genes used for the detection of Sporothrix humicola.

Target gene	Sequence (5′-3′)	PCR program†	Reference
ITS1-5.8S-ITS2 (rRNA gene)	ITS-1: TCC GTA GGT GAA CCT GCG G ITS-4: TCC TCC GCT TAT TGA TAT GC	1 1	[11]
beta-tubulin	bt2a: GGT AAC CAA ATC GGT GCT GCT TTC bt2b: ACC CTC AGT GTA GTG ACC CTT GGC	2 2	[17]
calmodulin	CL1: GAR TWC AAG GAG GCC TTC TC CL2: TTT TTG CAT CAT GAG TTG GAC	3 3	[18]

^†: PCR program.

1: ×1 (95 °C, 900 secs), ×40 (95 °C, 60s, 53 °C, 30s, 72 °C, 90s), ×1 (72 °C, 600 secs).

2: ×1 (94 °C, 240 secs), ×40 (94 °C, 45s, 58 °C, 45s, 72 °C, 90s), ×1 (72 °C, 600 secs).

3: ×1 (95 °C, 240 secs), ×40 (95 °C, 45s, 54 °C, 45s, 72 °C, 90s), ×1 (72 °C, 600 secs).