Table 3.
Growth and Development record of flies with different NPs at a concentration of 100 μg/ml.
| Type of NPs/ No. of days | Control | Green ZnO(100 μg/ml) | Chemical ZnO(100 μg/ml) | GO NPs (100 μg/ml) |
|---|---|---|---|---|
| Day 1 | Egg hatches | No egg | No egg | No egg |
| Day 2 | 1st instar observed | Egg hatches | No egg could be observed | Egg hatches |
| Day 3 | Some 2nd instar were observed along with 1st instar | 1st instar along with very few 2nd instar observed | Egg hatches | 1st instar observed |
| Day4 | Few 3rd instar larvae could be seen crawling in the food | Few 3rd instar larvae observed | 1 dead fly along with 1st and few 2nd instar observed | 2nd instar could be observed |
| Day 5 | Pupa formation observed | Many 2nd and 3rd instar larvae could be seen + very few pupa | Very few 3rd instar with many 1st and 2nd instar, 1 pupa also observed | 3rd instar larvae could be seen + few pupa observed |
| Day 6 | Pupa entering into adult | Many more new pupae seen | 3rd instar larvae and few pupa seen | Many pupae could be seen |
| Day 7 | Ready to emerge from pupa case | Adult | Many more new pupae seen | Ready to emerge from pupa case |
| Day 8 | Adult | – | Pupa developing | Adult |
| Day 9 | – | – | Adult | – |