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. 2019 Jun 27;30(9):1790–1800. doi: 10.1007/s13361-019-02254-y

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Distribution of cholesteryl linoleate (m/z 671.57) and cholesteryl oleate (m/z 673.59). (a) MALDI MSI of CE 18:2 in autopsy sample, P1–1, and P2. (b) MALDI MSI of CE 18:1 in autopsy sample, P1–1, and P2. c Box plot of maximum intensity (99th percentile) cholesteryl linoleate (CE 18:2), with over-expression in advanced plaques (P1 and P2), *p value < 0.0005. (d) Box plot of maximum intensity (99th percentile) cholesteryl oleate (CE 18:1), with over-expression in early plaque (autopsy), *p value < 0.0005. (e) Oil Red O staining of MALDI MSI section, showing fatty streak. (f) Immunohistochemistry CD68 stain (zoom, adjacent section), showing macrophages in fatty streak. (g) HE stain (zoom, adjacent section), showing fatty streak