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. 2019 Aug 15;9:11929. doi: 10.1038/s41598-019-47457-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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P. aeruginosa QS molecules disrupt bronchial epithelial cell bioenergetics and metabolic potential. BEAS-2B cells were treated with vehicle control (DMSO) or 100 μM 3-oxo-C12-HSL for 6 hours. Cells were then analyzed using Seahorse Cell Energy Phenotype assay. This allows for the real time measurement of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), which are representative of mitochondrial respiration and glycolysis respectively, at rest and after induction of a bioenergetic stress caused by treatment with the ATP synthase inhibitor, oligomycin, and the uncoupling agent, FCCP. (A) Plot of baseline and stressed conditions in control and 3-oxo-C12-HSL-treated cells. 3-oxo-C12-HSL-treated cells are more quiescent at baseline and have less metabolic potential under stressed conditions. (B) OCR measurements at baseline and stressed conditions. 3-oxo-C12-HSL decreases basal and stressed OCR as compared to control. (C) ECAR measurements at baseline and stressed conditions. 3-oxo-C12-HSL decreases basal and stressed ECAR as compared to control. Results are mean ± SEM. *p < 0.05, n = 3 independent experiments. One-way ANOVA with Tukey’s multiple comparisons test used for statistical analysis.