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. 2019 Aug 15;9:11923. doi: 10.1038/s41598-019-48433-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Yeast two-hybrid assay. Images depict representative colonies of S. cerevisiae expressing pairs of bait and prey fusion proteins, which are described at the left side of the panel. Each column represents a plate composition; DDO = double drop out (minus tryptophan and leucine), where no reporter gene is activated; QDO = quadruple drop out (minus tryptophan, leucine, histidine, and adenine), where two reporters are activated; and QDO/X/A = quadruple drop out with aurobasidin and X-α-Gal, where all four reporters are active. + represents the positive and − the negative control provided by Match Maker kit (Clontech).