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. 2019 Aug 15;9:11910. doi: 10.1038/s41598-019-48447-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression pattern analysis of R-10G epitope and vimentin in H9 cells grown on vitronectin and Matrigel. (a) Immunohistochemistry with no permeabilisation using antibodies for a pluripotency marker (R-10G epitope) and a mesenchymal marker (vimentin [VIM]). Nuclei were counterstained with DAPI. The expression levels of R-10G epitope and VIM were classified as “++” and “+” or “+” and “−”. The scale bar represents 200 µm. (b) Colony distributions for R-10G epitope and VIM expression patterns. The colonies were manually counted (n = 100, in triplicate). Values are the means ± standard errors. *** indicates P < 0.001 by unpaired two-tailed t-tests. R-10G++/VIM− and R-10G+/VIM + colonies were classified as “substate 1” and “substate 2”, respectively.