Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 15;9:11910. doi: 10.1038/s41598-019-48447-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Flow cytometry and immunohistochemical analyses in the H9 cell cultured on Matrigel. (a–c) Histograms of the H9 cells incubated with anti-vimentin antibodies (solid line) or isotype control (dash line). The substate 1 and substate 2 cells were separated from approximately 20% on the left and right side of vimentin signals, respectively (a). Flow cytometry analyses of substate 1 and substate 2 passaged one time after sorting (b,c). Data are representative of three independent experiments. (d) Immunohistochemistry with no permeabilisation using antibodies for a pluripotency marker (R-10G epitope) and a mesenchymal marker (vimentin [VIM]). Nuclei were counterstained with DAPI. The scale bar represents 200 µm.