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. 2019 Aug 15;9:11910. doi: 10.1038/s41598-019-48447-z

Figure 3.

Figure 3

Immunohistochemical analysis of pluripotency, differentiation, and epithelial-to-mesenchymal transition markers in H9 cells cultured on Matrigel. (a) Immunohistochemistry with no permeabilisation using antibodies for vimentin (VIM), SSEA-4, TRA-1-60, R-10G, E-cadherin (E-cad), and SSEA-1. (b) Immunohistochemistry with permeabilisation using antibodies for VIM, OCT3/4, NANOG, ZEB1, SNAI1/2, and TWIST. Nuclei were counterstained with DAPI. The inset shows a 3 × enlargement of the figure. The scale bar represents 200 µm.