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. 2019 Aug 15;9:11898. doi: 10.1038/s41598-019-47357-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mp_vATPase-like_dsRNA fed to M. persicae in artificial diet does not affect size or cause a knockdown of the target, even when delivered with transfection agents. (a) Quantitative real-time PCR showing that MP_vATPase-like transcripts level are not significantly different whether the aphids are fed Mp_vATPase-like_dsRNA or GFP_dsRNA. The fold change is not significantly different regardless of the transfection reagent used (ANOVA, p = 0.96, reps = 3). Total number of insects present in each replicate GFP_dsRNA - 4, 10, 8, Mp_vATPase – 4, 7, 8. (b) Aphid size does not differ between those fed on a diet containing Mp_vATPase-like_dsRNA and those fed GFP_dsRNA (Student t-test, p = 0.24), although use of the transfection agent does affect aphid size (Happyfect: p = 0.38, Lipofectamine: p = 0.94). The error bars show the standard error of the mean based on replicates = 3 replicates of 10 insects.