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. 2019 Aug 15;9:11922. doi: 10.1038/s41598-019-47462-4

Figure 6.

Figure 6

Involvement of SNX33 in effects of donepezil in primary cortical cells. (a,b) Effect of antisense MOs on SNX33 expression. Cells were treated with 1 μM control or antisense MOs, in addition to 6 μL/mL of Endo Porter (EP) for 24 h, and 48 h later, the cells were harvested. Cell lysate was subjected to western blotting. Representative images of western blotting analysis (a) and quantification of SNX33/β-actin ratio (b) are shown. ***P < 0.001 compared with control cells. n.s., not significant. Data are expressed as mean ± SEM of n = 5 independent observations. (c,d) Effect of SNX33 knockdown on donepezil-induced SNX33 expression. Cells were pretreated with MOs and EP for 24 h prior to 10 μM donepezil treatment for 48 h. Cell lysate was subjected to western blotting. Representative images of western blotting analysis (b) and quantification of SNX33/β-actin ratio (c) are shown. *P < 0.05; n.s., not significant. Data are expressed as mean ± SEM of n = 12–18 independent observations. SNX33, sorting nexin protein 33; MO, morpholino oligo.