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. 2019 Aug 15;9:11914. doi: 10.1038/s41598-019-48316-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Secretion of contraction-inducible myokines from hybrid myotubes comprised of C2C12 cells and human muscle cells obtained from a biopsy sample. After 7–8 days of differentiation, the differentiated myotubes originating from murine C2C12 cells and human primary myoblasts obtained from a biopsy sample (seeded in the 4 individual wells of 2 distinct culture plates) were treated with (n = 4) or without (n = 4) EPS at 20 V/25 mm, 1 Hz, 4-ms duration for 16 h. Conditioned media were collected and subjected to the 40-Plex BioPlex assay (*P < 0.05, n = 4). Two independent experiments using the same cell stock sample were performed, and representative results are shown. The Movement Index is also shown in the BOX.