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. 2019 Aug 15;9:11917. doi: 10.1038/s41598-019-48436-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Clustering of the SØ12 VRE isolates. (a) Dendrogram of SmaI macro-restriction pattern resolved in PFGE of VRE E. faecium with vanA. The UPGMA tree illustrates the distance between isolates. The two boxes show the two main clusters of isolates displaying >88% similarity, while the pulsotypes defined with >94% similarity and visual inspection are differentiated with colors and number. Pulsotype 1.1–1.5 (green/blue) belongs to cluster 1, while pulsotype 2.1–2.5 (red/pink/brown) belongs to cluster 2. The index case strain (ID 1) is highlighted with a yellow box. (b) Dendrogram of the same isolates of VREfm resolved with MALDI-TOF MS. The two boxes indicate the two main clusters identified with >90% similarity. The pulsotypes are assigned by PFGE in (a).