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. 2019 Aug 15;9:11878. doi: 10.1038/s41598-019-48047-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Rapamacyin-induced autophagy restores correct autophagic flux in KO cells after 72 h of treatment. (A) Western blotting analysis of the autophagy markers LC3 and p62 in WT and KO cells treated with different concentrations of the autophagy inducer rapamycin for up to 72 h. Full-length blots are presented in the Supplementary Information 5. (B) Trend of KO vs WT cells in rapamycin treatment at 72 h showed effective autophagic flux rescue. Data shown in this figure were reproduced independently three times. ns, not statistically significant; *p < 0.05; **p < 0.01.