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Aggresome staining in WT and sacsin KO cells. Representative images of p62 (in green) and aggresomes (in red) in WT and sacsin KO cells under normal conditions (A,C) or in WT cells after MG-132 treatment (B). Hoechst 33342 (in blue) was used as nuclear stain. Colocalization between aggresomes and p62 was absent in sacsin KO cells. Colocalization (in yellow) is indicated by arrows. Scale bar = 10 µm.
